Formulation of in-Vivo Experimental Model of Oral Submucous Fibrosis in Wistar Rat

Background: Oral submucous fibrosis is a chronic, fibrotic and crippling disease that affects the oral mucosa. It leads to progressive mainly involves buccal mucosa but other parts of cavity such as tongue palate can also be affected. There general consensus areca nut its constituents are responsible for pathogenesis disease. On histological examination biopsy taken from human mucosa, OSMF presents with atrophic epithelium juxta-epithelial deposition vast amount collagen. Material Methods:12 healthy male Wistar rats (age 5-6 weeks) were divided into two groups: group A (control) B (experimental) after ethical approval. Bleomycin an anti-cancer drug used create various animal models fibrosis. In this study, bleomycin was injected rat at concentration 1mg/mL dissolved in 0.01 M phosphate-buffered saline (PBS) 4-8 weeks daily. The control treated normal saline. Body weight mouth openings recorded. macroscopic done along microscopic analysis histopathological features immunohistochemical α-SMA expression. Masson’s trichrome stain visualize collagenous sub-epithelial tissue. Results: Clinically showed lesions mimicked OSMF. Changes lamina propria observed experimental compared group. At week 4, stratified squamous shortening rete ridges thick separate 8, became flat, loss collagen which completely hyalinized expression increased myofibroblasts. Conclusion: results our study suggested reproducible sustainable model Keywords: fibrosis, model, bleomycin, alpha-smooth muscle actin